Currently, α-ketoglutaric acid (AKG) is predominantly produced industrially via chemical synthesis. However, significant safety concerns in the production process limit its applications in food, cosmetics, feed, and other industries.
Fermentation methods for AKG production also face challenges such as low yield, difficulties in separation and purification, and lengthy production cycles, making them unsuitable for large-scale industrial production.
Shenzhen Xintianhe Biotechnology has developed an efficient AKG production process based on biotransformation, laying a technical foundation for industrial-scale AKG production.
CynbioX®AKG: Whole-Cell Catalysis Method-An Innovative Process Integrating Upstream and Downstream, Achieving Industry-Leading Standards
- Upstream: Constructed a dual-enzyme co-expression production strain for AKG, enabling one-step AKG production without exogenous enzyme intervention.
- Midstream: Developed a dual-enzyme cascade catalysis process, increasing AKG yield by 33% compared to the highest reported values and reducing production costs by over 50% compared to chemical synthesis.
- Downstream: Established a multi-stage membrane separation and purification process, achieving a product purity of ≥99.0%, meeting food-grade standards and surpassing the purity of traditionally chemically synthesized products.
The whole-cell catalysis method offers easily controllable production conditions, short production cycles, high raw material utilization, and minimal byproducts, positioning it at the forefront of domestic technology and enhancing competitiveness in both domestic and international markets.
A national patent has been granted for this innovation: "Glutamate Oxidase Mutant, Dual-Enzyme Co-Expression Vector, and Applications Thereof" (Patent Authorization No.: ZL 201910787949.3; Authorization Date: November 5, 2019).
Figure: Product Patent
CynbioX®AKG: Pharmaceutical Quality, Green and Safe
As an endogenous metabolite, CynbioX®AKG exhibits excellent biocompatibility. Shenzhen Xintianhe Biotechnology utilizes the whole-cell catalysis method to produce CynbioX®AKG with a purity exceeding 99%. The unique production process ensures fewer impurities, and no organic solvents are used during production, guaranteeing a greener and safer product. Experimental results confirm that CynbioX®AKG possesses higher biological activity and more defined efficacy.
Image:CynbioX®AKG series products.All rights reserved. Any infringement will be prosecuted
